MersV1, France, Analysis, bibRecord, 000402

Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo

Identifieur interne : 000402 ( France/Analysis ); précédent : 000401; suivant : 000403

Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo

Auteurs : Radhia Gargouri [France] ; Rajiv L. Joshi [France] ; John F. Bol [Pays-Bas] ; Suzanne Astier-Manifacier [France] ; Anne-Lise Haenni [France]

Source :

Virology [ 0042-6822 ] ; 1989.

RBID : ISTEX:88C4B9E0BD6AFE3ED99BAD16FC70020EF5541D06

English descriptors

Teeft :
- Agarose, Chinese cabbage, Denaturing, Denaturing conditions, Dsrna, Dsrnas, Ethanol, Ethanol precipitation, Ethidium bromide staining, Genome, Genomic, Genomic length, Genomic size, Glyoxal, Haenni, Hybridization, Internal initiation, Joshi, Methylation, Mosaic, Mosaic virus, Nascent, Nascent subgenomic, Native conditions, Northern blot hybridization, Nucleic, Nucleic acids, Nucleotide, Nucleotide sequence, Penultimate nucleoside, Polynucleotide kinase, Putative subgenomic dsrna, Replication, Replicative, Rna, Rnase, Room temperature, Subgenomic, Subgenomic length, Subgenomic rnas, Total tymv, Tracer, Turnip, Tymv, Tymv genomic, Uninfected, Vaccinia, Vaccinia virus, Vaccinia virus preparation, Viral, Virology.

Abstract

Abstract: Turnip yellow mosaic virus (TYMV) possesses a monopartite single-stranded (+) sense RNA genome in which the coat protein (cp) gene is 3′ proximal and is expressed in vivo via a subgenomic RNA. Evidence is presented here that this subgenomic RNA is synthesized in vivo by internal initiation of replication on (−) RNA strands of genomic length. The double-stranded RNAs (dsRNAs) from TYMV-infected plants have been isolated, purified, and characterized. Under native conditions, no dsRNAs (replicative intermeadites and/or replicative forms) of subgenomic length corresponding to subgenomic cp RNA can be detected by ethidium bromide staining of RNA-sizing gels or by Northern blot hybridization using RNA probes. The presence of nascent subgenomic cp (+) RNA strands on the dsRNA of genomic length has been demonstrated using two different approaches: (1) Northern blot hybridization using (−) RNA probes under denaturing conditions and (2) characterization of the 5′ ends of nascent (+) RNA strands upon labeling by vaccinia virus nucleoside-2′-methyltransferase.

Url:

https://api.istex.fr/ark:/67375/6H6-R36KM6SV-T/fulltext.pdf

DOI: 10.1016/0042-6822(89)90606-5

Affiliations:

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ISTEX:88C4B9E0BD6AFE3ED99BAD16FC70020EF5541D06

Le document en format XML

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<author><name sortKey="Joshi, Rajiv L" sort="Joshi, Rajiv L" uniqKey="Joshi R" first="Rajiv L." last="Joshi">Rajiv L. Joshi</name>
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<author><name sortKey="Bol, John F" sort="Bol, John F" uniqKey="Bol J" first="John F." last="Bol">John F. Bol</name>
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<author><name sortKey="Astier Manifacier, Suzanne" sort="Astier Manifacier, Suzanne" uniqKey="Astier Manifacier S" first="Suzanne" last="Astier-Manifacier">Suzanne Astier-Manifacier</name>
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<author><name sortKey="Astier Manifacier, Suzanne" sort="Astier Manifacier, Suzanne" uniqKey="Astier Manifacier S" first="Suzanne" last="Astier-Manifacier">Suzanne Astier-Manifacier</name>
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<term>Dsrna</term>
<term>Dsrnas</term>
<term>Ethanol</term>
<term>Ethanol precipitation</term>
<term>Ethidium bromide staining</term>
<term>Genome</term>
<term>Genomic</term>
<term>Genomic length</term>
<term>Genomic size</term>
<term>Glyoxal</term>
<term>Haenni</term>
<term>Hybridization</term>
<term>Internal initiation</term>
<term>Joshi</term>
<term>Methylation</term>
<term>Mosaic</term>
<term>Mosaic virus</term>
<term>Nascent</term>
<term>Nascent subgenomic</term>
<term>Native conditions</term>
<term>Northern blot hybridization</term>
<term>Nucleic</term>
<term>Nucleic acids</term>
<term>Nucleotide</term>
<term>Nucleotide sequence</term>
<term>Penultimate nucleoside</term>
<term>Polynucleotide kinase</term>
<term>Putative subgenomic dsrna</term>
<term>Replication</term>
<term>Replicative</term>
<term>Rna</term>
<term>Rnase</term>
<term>Room temperature</term>
<term>Subgenomic</term>
<term>Subgenomic length</term>
<term>Subgenomic rnas</term>
<term>Total tymv</term>
<term>Tracer</term>
<term>Turnip</term>
<term>Tymv</term>
<term>Tymv genomic</term>
<term>Uninfected</term>
<term>Vaccinia</term>
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<front><div type="abstract" xml:lang="en">Abstract: Turnip yellow mosaic virus (TYMV) possesses a monopartite single-stranded (+) sense RNA genome in which the coat protein (cp) gene is 3′ proximal and is expressed in vivo via a subgenomic RNA. Evidence is presented here that this subgenomic RNA is synthesized in vivo by internal initiation of replication on (−) RNA strands of genomic length. The double-stranded RNAs (dsRNAs) from TYMV-infected plants have been isolated, purified, and characterized. Under native conditions, no dsRNAs (replicative intermeadites and/or replicative forms) of subgenomic length corresponding to subgenomic cp RNA can be detected by ethidium bromide staining of RNA-sizing gels or by Northern blot hybridization using RNA probes. The presence of nascent subgenomic cp (+) RNA strands on the dsRNA of genomic length has been demonstrated using two different approaches: (1) Northern blot hybridization using (−) RNA probes under denaturing conditions and (2) characterization of the 5′ ends of nascent (+) RNA strands upon labeling by vaccinia virus nucleoside-2′-methyltransferase.</div>
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Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo

Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri